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    Induction of disease resistance by the plant activator, acibenzolar-S-methyl (ASM), against bacterial canker (Clavibacter michiganensis subsp. michiganensis) in tomato seedlings

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    Abstract
    The plant defence activator acibenzolar-S-methyl (benzo [1,2,3]thiadiazole-7-carbothioic acid-S-methyl ester, ASM; Bion 50 WG) was assayed on tomato seedlings for its ability to induce resistance against Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial canker of tomato. Pre-treatment of plants with ASM reduced the severity of the disease as well as the growth of the bacteria in planta. In ASM-treated plants, reduction in disease severity (up to 75%) was correlated with suppression of bacterial growth (up to 68.2%) during the time course of infection. In plants treated with ASM, activities of peroxidase (POX) and glutathione peroxidase (GPX) were determined as markers of resistance. Applications of ASM induced a progressive and significant increase of both enzymes in locally treated tissues. Such responses were expressed earlier and with a much higher magnitude when ASM-treated seedlings were challenged with the pathogen, thus providing support to the concept that a signal produced by the pathogen is essential for triggering enhanced synthesis and accumulation of these enzymes. No such activities were observed in water-treated control plants. Therefore, the slower symptom development and reduction in bacterial growth in ASM-treated plants might be due to the increase in activity of both oxidative and antioxidative protection systems in planta.
    Article Outline
    1. Introduction
    2. Materials and methods
    2.1. Plant material
    2.2. Bacterial strain and inoculation
    2.3. Application of ASM
    2.4. Effect of ASM on bacterial growth in planta
    2.5. Preparation of samples for determining enzyme activities
    2.6. Enzyme assays
    2.7. Experimental design and statistical analyses
    3. Results
    3.1. The effect of the ASM treatment on symptom development
    3.2. Bacterial multiplication in planta
    3.3. Changes in POX and GPX activities
    4. Discussion
    References
     

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    作者:Soylu, Soner, Baysal, ?mür, Soylu, E. Mine 来源:Elsevier 发布时间:2011年07月13日