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    Human gastric cells resistant to (-)-epigallocatechin gallate show cross-resistance to several environmental pollutants

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    Abstract
    After a long-term culture in (?)-epigallocatechin gallate (EGCG, 20 μM), a major constituent of green tea, human gastric AGS cells developed 2.2-fold resistance to EGCG. The resistant AGS (AGS-R) cells were cross-resistant to several N-methylcarbamate insecticides, which are among the major control agents for pest insects in Taiwan. The AGS-R cells also showed protective effects against both the cytotoxicity and DNA damage induced by one of the mutagenic derivatives of N-methylcarbamate insecticide, N-nitroso methomyl, which is known to target the mammalian gastric tract. Therefore, acquisition of resistance by AGS cells through chronic exposure to EGCG implies that the tea-drinking habit of the Taiwanese is probably beneficial for the health of the gastric tract. In addition, AGS-R cells were cross-resistant to sodium arsenite and hydrogen peroxide, indicating that tolerance to oxidative stress might play a role in the development of resistance described in this investigation.
    Article Outline
    1. Introduction
    2. Materials and methods
    2.1. Cells
    2.2. Chemicals
    2.3. Cell survival assays
    2.4. DNA damage assay
    2.5. Reduced glutathione (GSH) assay
    3. Results
    4. Discussion
    Acknowledgements
    References
     

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    作者:Horng, S.B., Kuo, H.H., Lin, M.Y., Lin, W.W., Wang, T.C. 来源:Elsevier 发布时间:2011年07月13日