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    Salicylic acid alleviated pathogen-induced oxidative stress in harvested sweet cherry fruit

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    Abstract
    The role of exogenous salicylic acid (SA) in regulating an antioxidative defense response of sweet cherry (Prunus avium L. cv. Hongdeng) fruit inoculated with Penicillium expansum was investigated by immunodetection of carbonylated proteins. After inoculation with P. expansum, carbonylated proteins accumulated to a lesser extent in SA-treated fruit than in control fruit, ranging from molecular mass 29–45 kDa. Higher activities of catalase (CAT), glutathione peroxidase (GPX), chitinase and β-1,3-glucanase were observed in SA-treated fruit. Similarly, the expressions of CAT, GPX and β-1,3-glucanase genes were also stimulated by SA treatment. Moreover, 2 mM SA did not inhibit P. expansum growth in vitro. These results indicate that SA activated antioxidant defense responses of sweet cherry fruit, which may play a role in the resistance against P. expansum.
    Article Outline
    1. Introduction
    2. Materials and methods
    2.1. Plant material
    2.2. Pathogen
    2.3. Biocontrol assay
    2.4. Effects of SA on mycelial growth and spore germination of P. expansum in vitro
    2.5. Preparation of total proteins and immunodetection of carbonylated proteins
    2.6. Enzyme assays
    2.7. Gene cloning by RT-PCR and RACE
    2.8. Isolation of total RNA and Northern blot analysis
    2.9. Statistical analysis
    3. Results
    3.1. Effects of SA on disease incidence and severity of blue mould
    3.2. Effects of SA on growth of P. expansum
    3.3. Immunoassay of carbonylated proteins
    3.4. Enzyme activity
    3.5. Gene cloning and expression analysis
    4. Discussion
    Acknowledgements
    References
     

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    作者:Xu, Xiangbin, Tian, Shiping 来源:Elsevier 发布时间:2011年07月13日