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    Altered juvenile hormone metabolism, reproduction and stress response in Drosophila adults with genetic ablation of the corpus allatum cells

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    Abstract
    Juvenile hormone (JH), which controls many developmental and physiological processes in Drosophila melanogaster, is synthesized de novo in the specialized endocrine glands, corpus allatum (CA). The present study concerns JH metabolism, reproduction and stress resistance in Drosophila with genetic ablation of a part of CA cells. The correlated regulation of JH biosynthesis and degradation in Drosophila adults has been found: ablation of CA cells led to (1) a dramatic decrease in activity of the key regulatory enzyme of JH biosynthesis, juvenile hormone acid methyl transferase and (2) a considerable increase in JH-hydrolyzing activity. It has been also shown that ablation of CA cells caused three significant physiological changes: (1) an increase in the intensity of response of JH degradation system to heat stress; (2) a disturbance of reproduction; (3) a decrease in stress resistance. Pharmacological rise of JH level rescued JH-hydrolyzing activity, fecundity and stress resistance in CA-ablated females. Pronouncedly, all the physiological effects caused by CA ablation were significant in females but not in males indicating a sexual dimorphism of JH physiological roles in Drosophila adults.Article Outline
    1. Introduction
    2. Materials and methods
    2.1. Drosophila strains and genetic experiments
    2.2. JHAMT activity assay
    2.3. JH-hydrolyzing activity assay
    2.4. JH treatment
    2.5. Heat stress treatment
    2.6. Ovary development estimation and fecundity analysis
    2.7. Viability analysis
    2.8. Statistical analysis
    3. Results
    3.1. Effects of genetic ablation of a part of CA cells on JHAMT activity
    3.2. Effects of CA ablation on JH degradation
    3.3. Effects of CA ablation on the response of JH degradation system to stress
    3.4. Effects of JH treatment on JH-hydrolyzing activity and JH stress reactivity in CA-ablated females
    3.5. Effects of CA ablation on reproduction
    3.6. Effects of CA ablation on stress resistance
    3.7. Effects of JH treatment on fecundity and stress resistance of CA-ablated females
    4. Discussion
    Acknowledgements
    References
     

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